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Last updated: 2016/12/4
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NEFL monoclonal antibody, clone 2G10

  • Catalog # : MAB10828
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NEFL.
  • Immunogen:
  • Recombinant protein corresponding to human NEFL.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.5% protein stabilizer, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) against HeLa (1) and Jurkat (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human brain (B) tissues using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4747
  • Gene Name:
  • NEFL
  • Gene Alias:
  • CMT1F,CMT2E,FLJ53642,NF-L,NF68,NFL
  • Gene Description:
  • neurofilament, light polypeptide
  • Gene Summary:
  • Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq
  • Other Designations:
  • light molecular weight neurofilament protein,neurofilament protein, light chain,neurofilament subunit NF-L,neurofilament triplet L protein,neurofilament, light polypeptide 68kDa,neurofilament-light
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