NEFL monoclonal antibody, clone 2G10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) against HeLa (1) and Jurkat (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human brain (B) tissues using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828).
Flow Cytometry
Flow cytometric analysis of Jurkat cells using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NEFL.
Immunogen
Recombinant protein corresponding to human NEFL.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) against HeLa (1) and Jurkat (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human brain (B) tissues using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828).Flow Cytometry
Flow cytometric analysis of Jurkat cells using NEFL monoclonal antibody, clone 2G10 (Cat # MAB10828) (green) and negative control (red). -
Gene Info — NEFL
Entrez GeneID
4747Gene Name
NEFL
Gene Alias
CMT1F, CMT2E, FLJ53642, NF-L, NF68, NFL
Gene Description
neurofilament, light polypeptide
Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq
Other Designations
light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|neurofilament-light
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Interactome
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Pathway
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Disease
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