NEDD8 monoclonal antibody, clone 5B8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) against C6 (1) and HeLa (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) against human NEDD8 (aa: 1-81) recombinant protein. (Expected MW is 40 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826).
Flow Cytometry
Flow cytometric analysis of HeLa cells using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NEDD8.
Immunogen
Recombinant protein corresponding to human NEDD8.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) against C6 (1) and HeLa (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) against human NEDD8 (aa: 1-81) recombinant protein. (Expected MW is 40 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826).Flow Cytometry
Flow cytometric analysis of HeLa cells using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) (green) and negative control (red). -
Gene Info — NEDD8
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Interactome
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