MYST1 monoclonal antibody, clone 8C4C4
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MYST1.
Immunogen
Recombinant protein corresponding to human MYST1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MYST1 monoclonal antibody, clone 8C4C4 (Cat # MAB10823) against HeLa (1), HepG2 (2) and SMMC-7721 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophageal squamous cell carcinoma (A), normal esophagus epithelium (B), rectum adenocarcinoma (C), lung squamous cell carcinoma (D), breast infiltrating carcinoma (E), and breast infiltrating carcinoma (F) tissues, showing nuclear localization using MYST1 monoclonal antibody, clone 8C4C4 (Cat # MAB10823) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of Eca-109 cells using MYST1 monoclonal antibody, clone 8C4C4 (Cat # MAB10823) (green), showing nuclear localization.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYST1
Entrez GeneID
84148Gene Name
MYST1
Gene Alias
FLJ14040, KAT8, MOF, hMOF
Gene Description
MYST histone acetyltransferase 1
Omim ID
609912Gene Ontology
HyperlinkGene Summary
The MYST family of histone acetyltransferases, which includes MYST1, is named for the founding members MOZ (MYST3; MIM 601408), yeast YBF2 and SAS2, and TIP60 (HTATIP; MIM 601409). All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin (Neal et al., 2000 [PubMed 10786633]).[supplied by OMIM
Other Designations
histone acetyltransferase MYST1|ortholog of Drosophila males absent on the first (MOF)
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Interactome
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Disease
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