Western blot analysis of HeLa and HepG2 cell lysates (5 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with NPM1 monoclonal antibody , clone 5E3 (1 : 1000) (Cat # MAB1082). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM
nucleolar phosphoprotein B23,nucleophosmin 1,nucleophosmin/nucleoplasmin family, member 1,numatrin