MSH6 monoclonal antibody, clone 3E1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) against HEK293 (1), HCT116 (2), A-549 (3), A-431 (4), MCF-7 (5) and HepG2 (6) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) against human MSH6 (aa: 217-395) recombinant protein. (Expected MW is 160 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human rectum cancer (B) tissues using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MSH6.
Immunogen
Recombinant protein corresponding to human MSH6.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) against HEK293 (1), HCT116 (2), A-549 (3), A-431 (4), MCF-7 (5) and HepG2 (6) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) against human MSH6 (aa: 217-395) recombinant protein. (Expected MW is 160 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human rectum cancer (B) tissues using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813).Flow Cytometry
Flow cytometric analysis of MCF-7 cells using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) (green) and negative control (red). -
Gene Info — MSH6
Entrez GeneID
2956Gene Name
MSH6
Gene Alias
GTBP, HNPCC5, HSAP
Gene Description
mutS homolog 6 (E. coli)
Omim ID
600678Gene Ontology
HyperlinkGene Summary
This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer. [provided by RefSeq
Other Designations
G/T mismatch-binding protein|mutS homolog 6|sperm-associated protein
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Interactome
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Pathway
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Disease
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