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MSH2 monoclonal antibody, clone 1B3 (3A2B8C)

  • Catalog # : MAB10812
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MSH2.
  • Immunogen:
  • Recombinant protein corresponding to human MSH2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Monkey
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using MSH2 monoclonal antibody, clone 1B3 (3A2B8C) (Cat # MAB10812) against HeLa (1), A-549 (2), A-431 (3) and HEK293 (4) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer (A) and lung cancer (B) tissues, showing nuclear localization using MSH2 monoclonal antibody, clone 3A2B8C (Cat # MAB10812) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of HeLa cells using MSH2 monoclonal antibody, clone 1B3 (3A2B8C) (Cat # MAB10812) (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4436
  • Gene Name:
  • MSH2
  • Gene Alias:
  • COCA1,FCC1,HNPCC,HNPCC1,LCFS2
  • Gene Description:
  • mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
  • Gene Summary:
  • MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq
  • Other Designations:
  • mutS homolog 2
  • Related Disease
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