MMP9 monoclonal antibody, clone 5G3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissues using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MMP9.
Immunogen
Recombinant protein corresponding to human MMP9.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissues using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810).Flow Cytometry
Flow cytometric analysis of HeLa cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (blue) and negative control (red). -
Gene Info — MMP9
Entrez GeneID
4318Gene Name
MMP9
Gene Alias
CLG4B, GELB, MMP-9
Gene Description
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Omim ID
120361Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq
Other Designations
92kD type IV collagenase|OTTHUMP00000031674|gelatinase B|macrophage gelatinase|matrix metalloproteinase 9|matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kD
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Interactome
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Pathway
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Disease
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