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Last updated: 2016/12/4
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MMP9 monoclonal antibody, clone 5G3

  • Catalog # : MAB10810
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MMP9.
  • Immunogen:
  • Recombinant protein corresponding to human MMP9.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against recombinant protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human brain tissues using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NIH/3T3 cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (blue) and negative control (red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4318
  • Gene Name:
  • MMP9
  • Gene Alias:
  • CLG4B,GELB,MMP-9
  • Gene Description:
  • matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
  • Gene Summary:
  • Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq
  • Other Designations:
  • 92kD type IV collagenase,OTTHUMP00000031674,gelatinase B,macrophage gelatinase,matrix metalloproteinase 9,matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase),matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kD
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