MMP1 monoclonal antibody, clone 6A5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) against HEK293 (1) and MMP1-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and human kidney cancer tissues (B) using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MMP1.
Immunogen
Recombinant protein corresponding to human MMP1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) against HEK293 (1) and MMP1-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and human kidney cancer tissues (B) using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808).Flow Cytometry
Flow cytometric analysis of HeLa cells using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green) and negative control (purple). -
Gene Info — MMP1
Entrez GeneID
4312Gene Name
MMP1
Gene Alias
CLG, CLGN
Gene Description
matrix metallopeptidase 1 (interstitial collagenase)
Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq
Other Designations
fibroblast collagenase|interstitial collagenase|matrix metalloprotease 1|matrix metalloproteinase 1|matrix metalloproteinase 1 (interstitial collagenase)
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Interactome
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Pathway
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Disease
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