Mouse monoclonal antibody raised against partial recombinant MMP1.
Recombinant protein corresponding to human MMP1.
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and human kidney cancer tissues (B) using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) with DAB staining.
Immunofluorescence analysis of HeLa cells using MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP1 monoclonal antibody, clone 6A5 (Cat # MAB10808) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq