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Last updated: 2017/2/26
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MCM2 monoclonal antibody, clone 1E7

  • Catalog # : MAB10806
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MCM2.
  • Immunogen:
  • Recombinant protein corresponding to human MCM2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) against MCF-7 (1), HeLa (2), Jurkat (3), K-562 (4), HEK293 (5) and HEPG2 (6) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) against human MCM2 (aa: 16-232) recombinant protein. (Expected MW is 50.4 kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human colon cancer (B) tissues using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4171
  • Gene Name:
  • MCM2
  • Gene Alias:
  • BM28,CCNL1,CDCL1,D3S3194,KIAA0030,MGC10606,MITOTIN,cdc19
  • Gene Description:
  • minichromosome maintenance complex component 2
  • Gene Summary:
  • The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq
  • Other Designations:
  • DNA replication licensing factor MCM2,MCM2 minichromosome maintenance deficient 2, mitotin,cell devision cycle-like 1,cyclin-like 1,minichromosome maintenance deficient 2 (mitotin),nuclear protein BM28
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