MCM2 monoclonal antibody, clone 2B3

Catalog # MAB10805

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Size:100 uL
Price: USD $ 531.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K-562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysate.

Western Blot (Recombinant protein)
Application

Western Blot (Recombinant protein)

Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against human MCM2 (aa: 16-232) recombinant protein. (Expected MW is 50.4 kDa)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human lung cancer (B) tissues using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) with DAB staining.

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA measurement of MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805).

Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of HeLa cells using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) (blue) and negative control (red).

  • Specification

    Product Description

    Mouse monoclonal antibody raised against partial recombinant MCM2.

    Immunogen

    Recombinant protein corresponding to human MCM2.

    Host

    Mouse

    Reactivity

    Human, Monkey, Mouse, Rat

    Form

    Liquid

    Isotype

    IgG1

    Recommend Usage

    ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In ascites (0.03% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K-562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysate.

    Western Blot (Recombinant protein)

    Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against human MCM2 (aa: 16-232) recombinant protein. (Expected MW is 50.4 kDa)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human lung cancer (B) tissues using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) with DAB staining.

    Enzyme-linked Immunoabsorbent Assay

    ELISA measurement of MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805).

    Flow Cytometry

    Flow cytometric analysis of HeLa cells using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) (blue) and negative control (red).
  • Gene Info — MCM2

    Entrez GeneID

    4171

    Gene Name

    MCM2

    Gene Alias

    BM28, CCNL1, CDCL1, D3S3194, KIAA0030, MGC10606, MITOTIN, cdc19

    Gene Description

    minichromosome maintenance complex component 2

    Omim ID

    116945

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq

    Other Designations

    DNA replication licensing factor MCM2|MCM2 minichromosome maintenance deficient 2, mitotin|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|nuclear protein BM28

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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