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Last updated: 2016/12/4
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MBP monoclonal antibody, clone 2H9

  • Catalog # : MAB10804
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MBP.
  • Immunogen:
  • Recombinant protein corresponding to human MBP.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against human MBP (aa: 1-197) recombinant protein. (Expected MW is 47 kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human brain (A) and human cerebellum (B) tissues using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of MSCs cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of MBP monoclonal antibody, clone 2H9 (Cat # MAB10804).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green) and negative control (red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4155
  • Gene Name:
  • MBP
  • Gene Alias:
  • MGC99675
  • Gene Description:
  • myelin basic protein
  • Gene Summary:
  • The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq
  • Other Designations:
  • Golli-mbp,OTTHUMP00000174383,OTTHUMP00000174384,OTTHUMP00000174385,OTTHUMP00000174386
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