MBP monoclonal antibody, clone 2H9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against human MBP (aa: 1-197) recombinant protein. (Expected MW is 47 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain (A) and human cerebellum (B) tissues using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of MSCs cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MBP monoclonal antibody, clone 2H9 (Cat # MAB10804).
Flow Cytometry
Flow cytometric analysis of HepG2 cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MBP.
Immunogen
Recombinant protein corresponding to human MBP.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against human MBP (aa: 1-197) recombinant protein. (Expected MW is 47 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain (A) and human cerebellum (B) tissues using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) with DAB staining.Immunofluorescence
Immunofluorescence analysis of MSCs cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MBP monoclonal antibody, clone 2H9 (Cat # MAB10804).Flow Cytometry
Flow cytometric analysis of HepG2 cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green) and negative control (red). -
Gene Info — MBP
Entrez GeneID
4155Gene Name
MBP
Gene Alias
MGC99675
Gene Description
myelin basic protein
Omim ID
159430Gene Ontology
HyperlinkGene Summary
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq
Other Designations
Golli-mbp|OTTHUMP00000174383|OTTHUMP00000174384|OTTHUMP00000174385|OTTHUMP00000174386
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Interactome
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Disease
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