MAPK3 monoclonal antibody, clone 1E5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) against HeLa (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K-562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) against human MAPK3 (aa: 9-143) recombinant protein. (Expected MW is 40.8 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (A) and human bladder cancer (B) tissues using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAPK3.
Immunogen
Recombinant protein corresponding to human MAPK3.
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) against HeLa (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K-562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) against human MAPK3 (aa: 9-143) recombinant protein. (Expected MW is 40.8 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (A) and human bladder cancer (B) tissues using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800).Flow Cytometry
Flow cytometric analysis of HeLa cells using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (blue) and negative control (red). -
Gene Info — MAPK3
Entrez GeneID
5595Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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