MAPK1 monoclonal antibody, clone 4C11
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More Files
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Western Blot (Cell lysate)
Western blot analysis using MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) against HeLa (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A-549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A) and breast carcinoma (B) showing cytoplasmic localization using MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) with DAB staining.
Immunofluorescence
Confocal immunofluorescence analysis of Eca-109 cells using MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) (green). MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) (green).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAPK1.
Immunogen
Recombinant protein corresponding to human MAPK1.
Host
Mouse
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) against HeLa (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A-549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A) and breast carcinoma (B) showing cytoplasmic localization using MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of Eca-109 cells using MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) (green). MAPK1 monoclonal antibody, clone 4C11 (Cat # MAB10799) (green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK1
Entrez GeneID
5594Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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