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Last updated: 2016/12/4
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MAP3K5 monoclonal antibody, clone 2E4

  • Catalog # : MAB10798
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MAP3K5.
  • Immunogen:
  • Recombinant protein corresponding to human MAP3K5.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) against human MAP3K5 (aa: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and prostate tissues (B) using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green) and negative control (purple).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4217
  • Gene Name:
  • MAP3K5
  • Gene Alias:
  • ASK1,MAPKKK5,MEKK5
  • Gene Description:
  • mitogen-activated protein kinase kinase kinase 5
  • Gene Summary:
  • Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
  • Other Designations:
  • MAP/ERK kinase kinase 5,MAPK/ERK kinase kinase 5,OTTHUMP00000017275,apoptosis signal regulating kinase
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