MAP3K2 monoclonal antibody, clone 4B4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) against HEK293 (1) and MAP3K2-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) against recombinant protein.
Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAP3K2.
Immunogen
Recombinant protein corresponding to human MAP3K2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) against HEK293 (1) and MAP3K2-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) against recombinant protein.Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP3K2 monoclonal antibody, clone 4B4 (Cat # MAB10797). -
Gene Info — MAP3K2
Entrez GeneID
10746Gene Name
MAP3K2
Gene Alias
MEKK2, MEKK2B
Gene Description
mitogen-activated protein kinase kinase kinase 2
Omim ID
609487Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 2|MAPK/ERK kinase kinase 2|MEK kinase 2
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Interactome
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Pathway
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Disease
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