MAP2K7 monoclonal antibody, clone 4E5
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) against human MAP2K7 (aa: 7-178) recombinant protein. (Expected MW is 45.1 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human muscle (B) tissues using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAP2K7.
Immunogen
Recombinant protein corresponding to human MAP2K7.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) against human MAP2K7 (aa: 7-178) recombinant protein. (Expected MW is 45.1 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human muscle (B) tissues using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796).Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K7 monoclonal antibody, clone 4E5 (Cat # MAB10796) (green) and negative control (red). -
Gene Info — MAP2K7
Entrez GeneID
5609Gene Name
MAP2K7
Gene Alias
Jnkk2, MAPKK7, MKK7, PRKMK7
Gene Description
mitogen-activated protein kinase kinase 7
Omim ID
603014Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq
Other Designations
JNK kinase 2|JNK-activating kinase 2|MAP kinase kinase 7|OTTHUMP00000174397|c-Jun N-terminal kinase kinase 2|dual specificity mitogen-activated protein kinase kinase 7
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Interactome
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Pathway
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