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Last updated: 2017/12/10
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MAP2K4 monoclonal antibody, clone 5H4

  • Catalog # : MAB10793
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MAP2K4.
  • Immunogen:
  • Recombinant protein corresponding to human MAP2K4.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) against HepG2 (1), K-562 (2), and HEK293 (3) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human muscle tissues using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) with DAB staining.
  • ELISA
  • ELISA
  • ELISA measurement of MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6416
  • Gene Name:
  • MAP2K4
  • Gene Alias:
  • JNKK,JNKK1,MAPKK4,MEK4,MKK4,PRKMK4,SEK1,SERK1
  • Gene Description:
  • mitogen-activated protein kinase kinase 4
  • Gene Summary:
  • This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq
  • Other Designations:
  • JNK activating kinase 1,JNK-activated kinase 1,MAP kinase kinase 4,MAPK/ERK kinase 4,SAPK/ERK kinase 1,c-Jun N-terminal kinase kinase 1,dual specificity mitogen-activated protein kinase kinase 4
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