MAP2K4 monoclonal antibody, clone 5H4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) against HepG2 (1), K-562 (2), and HEK293 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human muscle tissues using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793).
Flow Cytometry
Flow cytometric analysis of K-562 cells using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAP2K4.
Immunogen
Recombinant protein corresponding to human MAP2K4.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) against HepG2 (1), K-562 (2), and HEK293 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human muscle tissues using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793).Flow Cytometry
Flow cytometric analysis of K-562 cells using MAP2K4 monoclonal antibody, clone 5H4 (Cat # MAB10793) (green) and negative control (purple). -
Gene Info — MAP2K4
Entrez GeneID
6416Gene Name
MAP2K4
Gene Alias
JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SEK1, SERK1
Gene Description
mitogen-activated protein kinase kinase 4
Omim ID
601335Gene Ontology
HyperlinkGene Summary
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq
Other Designations
JNK activating kinase 1|JNK-activated kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|dual specificity mitogen-activated protein kinase kinase 4
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Interactome
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Disease
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