GMNN monoclonal antibody, clone 2H7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysates (50 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GMNN monoclonal antibody , clone 2H7 (1 : 500) (Cat # MAB1079). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GMNN.
Immunogen
Recombinant protein corresponding to amino acids 1-209 of human GMNN.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysates (50 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GMNN monoclonal antibody , clone 2H7 (1 : 500) (Cat # MAB1079). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — GMNN
-
Interactome
-
Disease
-
Publication Reference
-
Aurora-A controls pre-replicative complex assembly and DNA replication by stabilizing geminin in mitosis.
Tsunematsu T, Takihara Y, Ishimaru N, Pagano M, Takata T, Kudo Y.
Nature Communications 2013 May; 4:1885.
Application：WB, Human, HeLa cells.
-
The regulation of embryonic patterning and DNA replication by geminin.
Pitulescu M, Kessel M, Luo L.
Cellular and Molecular Life Sciences 2005 Jul; 62(13):1425.
Application：WB, Human, HeLa cells, Mammalian cells.
-
The cell-cycle regulator geminin inhibits Hox function through direct and polycomb-mediated interactions.
Luo L, Yang X, Takihara Y, Knoetgen H, Kessel M.
Nature 2004 Feb; 427(6976):749.
Application：IF, WB-Tr, ChIP, Human, U2OS, A375 cells.
-
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
McGarry TJ, Kirschner MW.
Cell 1998 Jun; 93(6):1043.
Application：IF, WB-Ce, Human, Xenopus, XL177, HeLa cells.
-
Aurora-A controls pre-replicative complex assembly and DNA replication by stabilizing geminin in mitosis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com