GFP monoclonal antibody, clone 4B10B2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784) against extracts from HCC827 cells, untransfected (1) and transfected with GFP(2).
Western Blot (Recombinant protein)
Western blot analysis using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784) against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
Immunocytochemistry
Immunocytochemistry analysis of HCC827 cells, untransfected (A) or transfected with GFP (B) using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784).
Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GFP.
Immunogen
Recombinant protein corresponding to GFP.
Host
Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784) against extracts from HCC827 cells, untransfected (1) and transfected with GFP(2).Western Blot (Recombinant protein)
Western blot analysis using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784) against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).Immunocytochemistry
Immunocytochemistry analysis of HCC827 cells, untransfected (A) or transfected with GFP (B) using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP monoclonal antibody, clone 4B10B2 (Cat # MAB10784).
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