Mouse monoclonal antibody raised against partial recombinant KIR2DL1.
Immunogen:
Recombinant protein corresponding to amino acids 23-223 of human KIR2DL1.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG2a, kappa
Recommend Usage:
ELISA Flow Cytometry Immunocytochemistry Immunofluorescence Western Blot(1:500-1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: Jurkat cell lysate.
Western Blot (Recombinant protein)
Western blot analysis of recombinant human protein KIR2DL1 (Lane 1), KIR2DL3 (Lane 2), KIR2DS4 (Lane 3) and KIR2DL4 (Lane 4) (each 50 ng per well) were resolved by SDS - PAGE, transferred to PVDF membrane and probed with KIR2DL1 monoclonal antibody , clone 2F9 (1 : 500) (Cat # MAB1078). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with KIR2DL1 monoclonal antibody, clone 2F9 (Cat#MAB1078) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of Hep3B cells, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq