VHL monoclonal antibody, clone AT82B10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HepG2, (2) HeLa, (3) Raji, (4) Jurkat, (5) A549, (6) MCF7, (7) PC3 cell lysate.
Immunofluorescence
Immunofluorescent staining of Hep3B cell line with antibody. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant VHL.
Immunogen
Recombinant protein corresponding to amino acids 1-154 of human VHL.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HepG2, (2) HeLa, (3) Raji, (4) Jurkat, (5) A549, (6) MCF7, (7) PC3 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of Hep3B cell line with antibody. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — VHL
Entrez GeneID
7428GeneBank Accession#
NM_000551Protein Accession#
NP_000542Gene Name
VHL
Gene Alias
HRCA1, RCA1, VHL1
Gene Description
von Hippel-Lindau tumor suppressor
Gene Ontology
HyperlinkGene Summary
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
elongin binding protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Chemokine receptor CXCR4 downregulated by von Hippel-Lindau tumour suppressor pVHL.
Staller P, Sulitkova J, Lisztwan J, Moch H, Oakeley EJ, Krek W.
Nature 2003 Sep; 425(6955):307.
Application：WB, Human, A498 cells.
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The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis.
Maxwell PH, Wiesener MS, Chang GW, Clifford SC, Vaux EC, Cockman ME, Wykoff CC, Pugh CW, Maher ER, Ratcliffe PJ.
Nature 1999 May; 399(6733):271.
Application：IP, WB-Ce, WB-Tr, Human, HeLa, RCC4 cells.
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Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function.
Stebbins CE, Kaelin WG Jr, Pavletich NP.
Science 1999 Apr; 284(5413):455.
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Chemokine receptor CXCR4 downregulated by von Hippel-Lindau tumour suppressor pVHL.
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