Western blot analysis of HepG2 cell lysate was resolved by SDS - PAGE , transferred to PVDF membrane and probed with VHL monoclonal antibody , clone 52A11 (1 : 500) (Cat # MAB1074) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq