KRT5 monoclonal antibody, clone 7H8C4
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using KRT5 monoclonal antibody, clone 7H8C4 (Cat # MAB10727) against truncated KRT5 recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skin tissues using KRT5 monoclonal antibody, clone 7H8C4 (Cat # MAB10727) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A) ,normal hepatocyte (B) , colon adenocacinoma?, normal stomach tissue (D) , showing cytoplasmic and membrane localization using KRT5 monoclonal antibody, clone 7H8C4 (Cat # MAB10727) with DAB staining.
Immunofluorescence
Immunofluorescence staining of methanol-fixed Eca-109 (upper) and HepG2 (bottom) cells showing cytoplasmic localization.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KRT5.
Immunogen
Recombinant protein corresponding to human KRT5.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using KRT5 monoclonal antibody, clone 7H8C4 (Cat # MAB10727) against truncated KRT5 recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skin tissues using KRT5 monoclonal antibody, clone 7H8C4 (Cat # MAB10727) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A) ,normal hepatocyte (B) , colon adenocacinoma?, normal stomach tissue (D) , showing cytoplasmic and membrane localization using KRT5 monoclonal antibody, clone 7H8C4 (Cat # MAB10727) with DAB staining.Immunofluorescence
Immunofluorescence staining of methanol-fixed Eca-109 (upper) and HepG2 (bottom) cells showing cytoplasmic localization.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KRT5
Entrez GeneID
3852Gene Name
KRT5
Gene Alias
CK5, DDD, EBS2, K5, KRT5A
Gene Description
keratin 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
58 kda cytokeratin|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)|keratin, type II cytoskeletal 5
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Interactome
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Disease
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