KIT monoclonal antibody, clone 1C5
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KIT.
Immunogen
Recombinant protein corresponding to human KIT.
Host
Mouse
Theoretical MW (kDa)
145
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using KIT monoclonal antibody, clone 1C5 (Cat # MAB10712) against Jurkat (1) and HeLa (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using KIT monoclonal antibody, clone 1C5 (Cat # MAB10712) against human KIT (aa 805-976) recombinant protein. (Expected MW is 45 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human malignant stromal tumors tissues using KIT monoclonal antibody, clone 1C5 (Cat # MAB10712) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of KIT monoclonal antibody, clone 1C5 (Cat # MAB10712) . -
Gene Info — KIT
Entrez GeneID
3815Gene Name
KIT
Gene Alias
C-Kit, CD117, PBT, SCFR
Gene Description
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Gene Ontology
HyperlinkGene Summary
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
mast/stem cell growth factor receptor|proto-oncogene tyrosine-protein kinase Kit|soluble KIT variant 1
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Interactome
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Disease
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