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Last updated: 2020/8/2
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MIF monoclonal antibody, clone 4E5 

  • Catalog # : MAB1071
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinantMIF.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-115 of human MIF.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: THP-1 cell lysate; Lane 3: HeLa cell lysate; Lane 4: U937 cell lysate.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry analysis of Balb/3T3 cells. The cell was stained with MIF monoclonal antibody, clone 4E5 (Catalog # MAB1071) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells. The cell was stained with MIF monoclonal antibody, clone 4E5 (Catalog # MAB1071) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 4282
  • Gene Name:
  • MIF
  • Gene Alias:
  • GIF,GLIF,MMIF
  • Gene Description:
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • Gene Summary:
  • This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
  • Other Designations:
  • glycosylation-inhibiting factor,phenylpyruvate tautomerase
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