Western blot analysis of HL-60 extract was resolved by SDS - PAGE , transferred to PVDF membrane and probed with MIF monoclonal antibody , clone 4E4 (1 : 1000) (Cat # MAB1071) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq