MIF monoclonal antibody, clone 4E5
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: THP-1 cell lysate; Lane 3: HeLa cell lysate; Lane 4: U937 cell lysate.
Immunocytochemistry
Immunocytochemistry analysis of Balb/3T3 cells. The cell was stained with MIF monoclonal antibody, clone 4E5 (Catalog # MAB1071) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with MIF monoclonal antibody, clone 4E5 (Catalog # MAB1071) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinantMIF.
Immunogen
Recombinant protein corresponding to amino acids 1-115 of human MIF.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: THP-1 cell lysate; Lane 3: HeLa cell lysate; Lane 4: U937 cell lysate.Immunocytochemistry
Immunocytochemistry analysis of Balb/3T3 cells. The cell was stained with MIF monoclonal antibody, clone 4E5 (Catalog # MAB1071) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with MIF monoclonal antibody, clone 4E5 (Catalog # MAB1071) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MIF
Entrez GeneID
4282GeneBank Accession#
NM_002415Protein Accession#
NP_002406Gene Name
MIF
Gene Alias
GIF, GLIF, MMIF
Gene Description
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene Ontology
HyperlinkGene Summary
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
Other Designations
glycosylation-inhibiting factor|phenylpyruvate tautomerase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid-induced regulator of the immune response.
Bucala R.
FASEB Journal 1996 Dec; 10(14):1607.
Application：ELISA, Human, Mouse, T lymphocytes.
-
Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF).
Bernhagen J, Mitchell RA, Calandra T, Voelter W, Cerami A, Bucala R.
Biochemistry 1994 Nov; 3(47):14144.
Application：Func, WB-Re, Mouse, L929 cells, Purified proteins, Raw 264.7 cells.
-
Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.
Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR.
PNAS 1989 Oct; 86(19):7522.
-
MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid-induced regulator of the immune response.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com