JMJD2A monoclonal antibody, clone 5H1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (upper) and human larynx cancer tissues (bottom) using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant JMJD2A.
Immunogen
Recombinant protein corresponding to human JMJD2A.
Host
Mouse
Theoretical MW (kDa)
120
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (upper) and human larynx cancer tissues (bottom) using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using JMJD2A monoclonal antibody, clone 5H1 (Cat # MAB10708) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — JMJD2A
Entrez GeneID
9682Gene Name
JMJD2A
Gene Alias
JHDM3A, JMJD2, KDM4A, KIAA0677
Gene Description
jumonji domain containing 2A
Omim ID
609764Gene Ontology
HyperlinkGene Summary
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq
Other Designations
OTTHUMP00000008810|jumonji C domain-containing histone demethylase 3A
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