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Last updated: 2017/8/20
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JAK3 monoclonal antibody, clone 5H2

  • Catalog # : MAB10705
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant JAK3.
  • Immunogen:
  • Recombinant protein corresponding to human JAK3.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 125
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) against Jurkat cell lysate.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (blue) and negative control (red) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3718
  • Gene Name:
  • JAK3
  • Gene Alias:
  • JAK-3,JAK3_HUMAN,JAKL,L-JAK,LJAK
  • Gene Description:
  • Janus kinase 3 (a protein tyrosine kinase, leukocyte)
  • Gene Summary:
  • The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq
  • Other Designations:
  • Janus kinase 3,leukocyte Janus kinase,tyrosine-protein kinase JAK3
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