ITK monoclonal antibody, clone 5G6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) against Jurkat cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) against recombinant protein.
Immunofluorescence
Immunofluorescence analysis of Jurkat cells using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) (green) . Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) .
Flow Cytometry
Flow cytometric analysis of Jurkat cells using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ITK.
Immunogen
Recombinant protein corresponding to human ITK.
Host
Mouse
Theoretical MW (kDa)
72
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) against Jurkat cell lysate.Western Blot (Recombinant protein)
Western blot analysis using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) against recombinant protein.Immunofluorescence
Immunofluorescence analysis of Jurkat cells using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) .Flow Cytometry
Flow cytometric analysis of Jurkat cells using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) (blue) and negative control (red). -
Gene Info — ITK
Entrez GeneID
3702Gene Name
ITK
Gene Alias
EMT, LYK, MGC126257, MGC126258, PSCTK2
Gene Description
IL2-inducible T-cell kinase
Omim ID
186973Gene Ontology
HyperlinkGene Summary
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq
Other Designations
homolog of mouse T-cell itk/tsk|tyrosine-protein kinase ITK/TSK|tyrosine-protein kinase LYK
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Interactome
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Pathway
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Disease
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