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Last updated: 2016/12/4
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ITK monoclonal antibody, clone 5G6

  • Catalog # : MAB10704
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ITK.
  • Immunogen:
  • Recombinant protein corresponding to human ITK.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 72
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) against Jurkat cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) against recombinant protein.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of Jurkat cells using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) (green) . Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • ELISA
  • ELISA measurement of ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) .
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells using ITK monoclonal antibody, clone 5G6 (Cat # MAB10704) (blue) and negative control (red) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3702
  • Gene Name:
  • ITK
  • Gene Alias:
  • EMT,LYK,MGC126257,MGC126258,PSCTK2
  • Gene Description:
  • IL2-inducible T-cell kinase
  • Gene Summary:
  • This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq
  • Other Designations:
  • homolog of mouse T-cell itk/tsk,tyrosine-protein kinase ITK/TSK,tyrosine-protein kinase LYK
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