ITGA5 monoclonal antibody, clone 10F6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using ITGA5 monoclonal antibody, clone 10F6 (Cat # MAB10703) against ITGA5-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using ITGA5 monoclonal antibody, clone 10F6 (Cat # MAB10703) .
Flow Cytometry
Flow cytometric analysis of HeLa cells using ITGA5 monoclonal antibody, clone 10F6 (Cat # MAB10703) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ITGA5.
Immunogen
Recombinant protein corresponding to human ITGA5.
Host
Mouse
Theoretical MW (kDa)
114
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using ITGA5 monoclonal antibody, clone 10F6 (Cat # MAB10703) against ITGA5-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using ITGA5 monoclonal antibody, clone 10F6 (Cat # MAB10703) .Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using ITGA5 monoclonal antibody, clone 10F6 (Cat # MAB10703) (green) and negative control (purple). -
Gene Info — ITGA5
Entrez GeneID
3678Gene Name
ITGA5
Gene Alias
CD49e, FNRA, VLA5A
Gene Description
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Omim ID
135620Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq
Other Designations
fibronectin receptor, alpha subunit|integrin alpha 5|very late activation protein 5, alpha subunit
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Interactome
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Pathway
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Disease
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