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Last updated: 2017/3/26

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ISL1 monoclonal antibody, clone 1H9

  • Catalog # : MAB10702
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ISL1.
  • Immunogen:
  • Recombinant protein corresponding to human ISL1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 39
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) against full-length ISL1 (aa 1-349) -hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and cervical carcinoma (bottom) , showing nuclear localization using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) (green) . Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3670
  • Gene Name:
  • ISL1
  • Gene Alias:
  • Isl-1
  • Gene Description:
  • ISL LIM homeobox 1
  • Gene Summary:
  • This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
  • Other Designations:
  • ISL1 transcription factor, LIM/homeodomain,islet-1
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