IRAK4 monoclonal antibody, clone 2H9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) against THP-1 (1) , HeLa (2) , K-562 (3) , MCF-7 (4) , RAW264.7 (5) , Jurkat (6) and COS-7 (7) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) against recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human kidney cancer tissues using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) .
Flow Cytometry
Flow cytometric analysis of HeLa cells using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant IRAK4.
Immunogen
Recombinant protein corresponding to human IRAK4.
Host
Mouse
Theoretical MW (kDa)
52
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) against THP-1 (1) , HeLa (2) , K-562 (3) , MCF-7 (4) , RAW264.7 (5) , Jurkat (6) and COS-7 (7) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) against recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human kidney cancer tissues using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) .Flow Cytometry
Flow cytometric analysis of HeLa cells using IRAK4 monoclonal antibody, clone 2H9 (Cat # MAB10701) (blue) and negative control (red). -
Gene Info — IRAK4
Entrez GeneID
51135Gene Name
IRAK4
Gene Alias
IPD1, NY-REN-64, REN64
Gene Description
interleukin-1 receptor-associated kinase 4
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
Other Designations
IRAK-4 mutated form 1|interleukin-1 receptor associated kinase 4
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Interactome
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Pathway
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Disease
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