Western blot analysis of 293T , MCF - 7 , HT - 29 , HL - 60 , HeLa , mouse brain , and rat brain extracts were resolved by SDS - PAGE , transferred to PVDF membrane and probed with HTRA2 monoclonal antibody , clone 1B3 (1 : 1000) (Cat # MAB1070) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
HtrA-like serine protease,Omi stress-regulated endoprotease,high temperature requirement protein A2,protease, serine, 25