IL34 monoclonal antibody, clone 1D12
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant IL34.
Immunogen
Recombinant protein corresponding to human IL34.
Host
Mouse
Theoretical MW (kDa)
27.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using IL34 monoclonal antibody, clone 1D12 (Cat # MAB10698) against IL34-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence analysis of U251 cells using IL34 monoclonal antibody, clone 1D12 (Cat # MAB10698) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using IL34 monoclonal antibody, clone 1D12 (Cat # MAB10698) (green) and negative control (purple) . -
Gene Info — IL34
Entrez GeneID
146433Gene Name
IL34
Gene Alias
C16orf77, IL-34, MGC34647
Gene Description
interleukin 34
Gene Ontology
HyperlinkGene Summary
Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM
Other Designations
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