HK1 monoclonal antibody, clone 3A10
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HK1.
Immunogen
Recombinant protein corresponding to human HK1.
Host
Mouse
Theoretical MW (kDa)
120
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using HK1 monoclonal antibody, clone 3A10 (Cat # MAB10683) against Jurkat (1) , HeLa (2) , HepG2 (3) and NIH/3T3 (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissues using HK1 monoclonal antibody, clone 3A10 (Cat # MAB10683) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using HK1 monoclonal antibody, clone 3A10 (Cat # MAB10683) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using HK1 monoclonal antibody, clone 3A10 (Cat # MAB10683) (green) and negative control (purple). -
Gene Info — HK1
Entrez GeneID
3098Gene Name
HK1
Gene Alias
HK1-ta, HK1-tb, HK1-tc, HKI, HXK1
Gene Description
hexokinase 1
Omim ID
142600Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq
Other Designations
OTTHUMP00000019725|brain form hexokinase|glycolytic enzyme
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
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Disease
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Publication Reference
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Overexpression of Protein Phosphatase 2 Regulatory Subunit B"Alpha Promotes Glycolysis by Regulating Hexokinase 1 in Hepatocellular Carcinoma.
Ning Jiao, Wan Sheng Ji, Biao Zhang, Yu Kui Shang, Yu Chen Zhang, Wei Qun Yu, Hai Long Jin, Chao Li, Cheng Ying Zhang, Cheng Yan, Wen Yue, Qing Zhang.
Biomedical and Environmental Sciences 2022 Jul; 35(7):622.
Application:IF, Human, HepG2, Huh7 cells.
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Overexpression of Protein Phosphatase 2 Regulatory Subunit B"Alpha Promotes Glycolysis by Regulating Hexokinase 1 in Hepatocellular Carcinoma.
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