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HK1 monoclonal antibody, clone 7A7

  • Catalog # : MAB10682
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HK1.
  • Immunogen:
  • Recombinant protein corresponding to human HK1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 102
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using HK1 monoclonal antibody, clone 7A7 (Cat # MAB10682) against Jurkat (1) , HeLa (2) , HepG2 (3) , MCF-7 (4) and PC-12 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human salivary gland tissues (upper) and kidney tissues (bottom) using HK1 monoclonal antibody, clone 7A7 (Cat # MAB10682) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NIH/3T3 cells using HK1 monoclonal antibody, clone 7A7 (Cat # MAB10682) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3098
  • Gene Name:
  • HK1
  • Gene Alias:
  • HK1-ta,HK1-tb,HK1-tc,HKI,HXK1
  • Gene Description:
  • hexokinase 1
  • Gene Summary:
  • Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019725,brain form hexokinase,glycolytic enzyme
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