HIF1A monoclonal antibody, clone 1A3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) against COS-7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) , lung cancer tissues (B) , stomach cancer tissues (C) and brain tumor tissues (D) using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) .
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HIF1A.
Immunogen
Recombinant protein corresponding to human HIF1A.
Host
Mouse
Theoretical MW (kDa)
120
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) against COS-7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) , lung cancer tissues (B) , stomach cancer tissues (C) and brain tumor tissues (D) using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) . -
Gene Info — HIF1A
Entrez GeneID
3091Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
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Interactome
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Pathway
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Disease
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