Product Browser

Last updated: 2016/11/27

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

HIF1A monoclonal antibody, clone 1A3

  • Catalog # : MAB10681
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HIF1A.
  • Immunogen:
  • Recombinant protein corresponding to human HIF1A.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 120
  • Reactivity:
  • Human, Monkey, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) against COS-7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) , lung cancer tissues (B) , stomach cancer tissues (C) and brain tumor tissues (D) using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3091
  • Gene Name:
  • HIF1A
  • Gene Alias:
  • HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
  • Gene Description:
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Gene Summary:
  • Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • ARNT interacting protein,hypoxia-inducible factor 1, alpha subunit,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1
  • RSS
  • YouTube
  • Linkedin
  • Facebook