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Last updated: 2016/11/27

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HFE monoclonal antibody, clone 3F1

  • Catalog # : MAB10680
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HFE.
  • Immunogen:
  • Recombinant protein corresponding to human HFE.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 40
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using HFE monoclonal antibody, clone 3F1 (Cat # MAB10680) against HEK293 (1) and HFE-hIgGFc transfected HEK293 (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using HFE monoclonal antibody, clone 3F1 (Cat # MAB10680) against recombinant protein.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using HFE monoclonal antibody, clone 3F1 (Cat # MAB10680) (green) . Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • ELISA
  • ELISA measurement of HFE monoclonal antibody, clone 3F1 (Cat # MAB10680) .
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3077
  • Gene Name:
  • HFE
  • Gene Alias:
  • HFE1,HH,HLA-H,MGC103790,dJ221C16.10.1
  • Gene Description:
  • hemochromatosis
  • Gene Summary:
  • The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq
  • Other Designations:
  • MHC class I-like protein HFE,hemochromatosis protein,hereditary hemochromatosis protein HLA-H
  • Related Disease
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