Western blot analysis of extracts of Ramos , THP - 1 , A20 and NIH/3T3 were resolved by SDS - PAGE , transferred to PVDF membrane and probed with IRF5 monoclonal antibody , clone 10T1 (1 : 1000) (Cat # MAB1068) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. [provided by RefSeq