HCK monoclonal antibody, clone 3D12E10
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More Files
- More Functions
Western Blot
Western blot analysis using HCK monoclonal antibody, clone 3D12E10 (Cat # MAB10679) against truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (upper) and ancreas cancer (bottom) , showing cytoplasmic localization using HCK monoclonal antibody, clone 3D12E10 (Cat # MAB10679) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HCK.
Immunogen
Recombinant protein corresponding to human HCK.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using HCK monoclonal antibody, clone 3D12E10 (Cat # MAB10679) against truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (upper) and ancreas cancer (bottom) , showing cytoplasmic localization using HCK monoclonal antibody, clone 3D12E10 (Cat # MAB10679) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HCK
Entrez GeneID
3055Gene Name
HCK
Gene Alias
JTK9
Gene Description
hemopoietic cell kinase
Omim ID
142370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq
Other Designations
tyrosine protein kinase HCK
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Interactome
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Pathway
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Disease
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