Western blot analysis using GOT2 monoclonal antibody, clone 3E9 (Cat # MAB10669) against HEK293 (1) , PC-12 (2) , HL-60 (3) , BCBL-1 (4) , HepG2 (5) and NIH/3T3 (6) cell lysate.
Immunofluorescence analysis of PC-3 (upper) and SK-BR-3 (bottom) cells using GOT2 monoclonal antibody, clone 3E9 (Cat # MAB10669) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
aspartate aminotransferase 2,kynurenine aminotransferase IV