GOT2 monoclonal antibody, clone 3E9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using GOT2 monoclonal antibody, clone 3E9 (Cat # MAB10669) against HEK293 (1) , PC-12 (2) , HL-60 (3) , BCBL-1 (4) , HepG2 (5) and NIH/3T3 (6) cell lysate.
Immunofluorescence
Immunofluorescence analysis of PC-3 (upper) and SK-BR-3 (bottom) cells using GOT2 monoclonal antibody, clone 3E9 (Cat # MAB10669) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GOT2.
Immunogen
Recombinant protein corresponding to human GOT2.
Host
Mouse
Theoretical MW (kDa)
47
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using GOT2 monoclonal antibody, clone 3E9 (Cat # MAB10669) against HEK293 (1) , PC-12 (2) , HL-60 (3) , BCBL-1 (4) , HepG2 (5) and NIH/3T3 (6) cell lysate.Immunofluorescence
Immunofluorescence analysis of PC-3 (upper) and SK-BR-3 (bottom) cells using GOT2 monoclonal antibody, clone 3E9 (Cat # MAB10669) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GOT2
Entrez GeneID
2806Gene Name
GOT2
Gene Alias
FLJ40994, KAT4, KATIV, mitAAT
Gene Description
glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Omim ID
138150Gene Ontology
HyperlinkGene Summary
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations
aspartate aminotransferase 2|kynurenine aminotransferase IV
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Interactome
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Pathway
- Alanine
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Cysteine and methionine metabolism
- Isoquinoline alkaloid biosynthesis
- Metabolic pathways
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