GFI1 monoclonal antibody, clone 5D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using GFI1 monoclonal antibody, clone 5D7 (Cat # MAB10668) against HEK293 (1) and GFI1-hIgGFc transfected HEK293 (2) cell lysate.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GFI1.
Immunogen
Recombinant protein corresponding to human GFI1.
Host
Mouse
Theoretical MW (kDa)
45
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using GFI1 monoclonal antibody, clone 5D7 (Cat # MAB10668) against HEK293 (1) and GFI1-hIgGFc transfected HEK293 (2) cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GFI1
Entrez GeneID
2672Gene Name
GFI1
Gene Alias
FLJ94509, GFI-1, ZNF163
Gene Description
growth factor independent 1 transcription repressor
Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000012582|OTTHUMP00000012583|growth factor independence-1|growth factor independent 1|zinc finger protein 163|zinc finger protein Gfi-1
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Interactome
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Disease
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