G6PD monoclonal antibody, clone 2H7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) against HeLa (1) , MCF-7 (2) , Jurkat (3) and K-562 (4) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) against human G6PD (aa 275-515) recombinant protein. (Expected MW is 53.1 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (upper) and human kidney cancer (bottom) tissues using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) .
Flow Cytometry
Flow cytometric analysis of Jurkat cells using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant G6PD.
Immunogen
Recombinant protein corresponding to human G6PD.
Host
Mouse
Theoretical MW (kDa)
59
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) against HeLa (1) , MCF-7 (2) , Jurkat (3) and K-562 (4) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) against human G6PD (aa 275-515) recombinant protein. (Expected MW is 53.1 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (upper) and human kidney cancer (bottom) tissues using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) .Flow Cytometry
Flow cytometric analysis of Jurkat cells using G6PD monoclonal antibody, clone 2H7 (Cat # MAB10661) (green) and negative control (red). -
Gene Info — G6PD
Entrez GeneID
2539Gene Name
G6PD
Gene Alias
G6PD1
Gene Description
glucose-6-phosphate dehydrogenase
Omim ID
305900Gene Ontology
HyperlinkGene Summary
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026034|glucose-6-phosphate 1-dehydrogenase|glucose-6-phosphate dehydrogenase, G6PD
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Interactome
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