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Last updated: 2016/11/27
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G6PD monoclonal antibody, clone 5E12

  • Catalog # : MAB10660
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant G6PD.
  • Immunogen:
  • Recombinant protein corresponding to human G6PD.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 59
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using G6PD monoclonal antibody, clone 5E12 (Cat # MAB10660) against HeLa (1) , MCF-7 (2) , Jurkat (3) and K-562 (4) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using G6PD monoclonal antibody, clone 5E12 (Cat # MAB10660) against human G6PD (aa 275-515) recombinant protein. (Expected MW is 53.1 kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human ovarian cancer (upper) and human stomach cancer (bottom) tissues using G6PD monoclonal antibody, clone 5E12 (Cat # MAB10660) with DAB staining.
  • ELISA
  • ELISA
  • ELISA measurement of G6PD monoclonal antibody, clone 5E12 (Cat # MAB10660) .
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using G6PD monoclonal antibody, clone 5E12 (Cat # MAB10660) (green) and negative control (red) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2539
  • Gene Name:
  • G6PD
  • Gene Alias:
  • G6PD1
  • Gene Description:
  • glucose-6-phosphate dehydrogenase
  • Gene Summary:
  • This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000026034,glucose-6-phosphate 1-dehydrogenase,glucose-6-phosphate dehydrogenase, G6PD
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