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Last updated: 2017/9/17
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FYN monoclonal antibody, clone 2A10

  • Catalog # : MAB10658
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant FYN.
  • Immunogen:
  • Recombinant protein corresponding to human FYN.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 61
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) against HeLa cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (upper) and brain tissues (bottom) using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of U251 cells using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) .
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) (green) and negative control (purple) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2534
  • Gene Name:
  • FYN
  • Gene Alias:
  • MGC45350,SLK,SYN
  • Gene Description:
  • FYN oncogene related to SRC, FGR, YES
  • Gene Summary:
  • This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq
  • Other Designations:
  • OKT3-induced calcium influx regulator,OTTHUMP00000017914,OTTHUMP00000017915,OTTHUMP00000017917,c-syn protooncogene,protein-tyrosine kinase fyn,proto-oncogene tyrosine-protein kinase fyn,src-like kinase,src/yes-related novel,tyrosine kinase p59fyn(T)
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