FYN monoclonal antibody, clone 2A10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) against HeLa cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (upper) and brain tissues (bottom) using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of U251 cells using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) .
Flow Cytometry
Flow cytometric analysis of HeLa cells using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FYN.
Immunogen
Recombinant protein corresponding to human FYN.
Host
Mouse
Theoretical MW (kDa)
61
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) against HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (upper) and brain tissues (bottom) using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) with DAB staining.Immunofluorescence
Immunofluorescence analysis of U251 cells using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) .Flow Cytometry
Flow cytometric analysis of HeLa cells using FYN monoclonal antibody, clone 2A10 (Cat # MAB10658) (green) and negative control (purple). -
Gene Info — FYN
Entrez GeneID
2534Gene Name
FYN
Gene Alias
MGC45350, SLK, SYN
Gene Description
FYN oncogene related to SRC, FGR, YES
Omim ID
137025Gene Ontology
HyperlinkGene Summary
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq
Other Designations
OKT3-induced calcium influx regulator|OTTHUMP00000017914|OTTHUMP00000017915|OTTHUMP00000017917|c-syn protooncogene|protein-tyrosine kinase fyn|proto-oncogene tyrosine-protein kinase fyn|src-like kinase|src/yes-related novel|tyrosine kinase p59fyn(T)
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Interactome
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Pathway
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Disease
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