FUK monoclonal antibody, clone 6E2
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FUK.
Immunogen
Recombinant protein corresponding to human FUK.
Host
Mouse
Theoretical MW (kDa)
117
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using FUK monoclonal antibody, clone 6E2 (Cat # MAB10657) against HeLa (1) , HepG2 (2) , Jurkat (3) , A-431 (4) , HEK293 (5) , MCF-7 (6) , PC-12 (7) , COS-7 (8) , and NIH/3T3 (9) cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of FUK monoclonal antibody, clone 6E2 (Cat # MAB10657) .Flow Cytometry
Flow cytometric analysis of HeLa cells using FUK monoclonal antibody, clone 6E2 (Cat # MAB10657) (green) and negative control (purple). -
Gene Info — FUK
Entrez GeneID
197258Gene Name
FUK
Gene Alias
1110046B12Rik, FLJ39408
Gene Description
fucokinase
Omim ID
608675Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq
Other Designations
L-fucose kinase|OTTHUMP00000082728
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