ETS1 monoclonal antibody, clone 8A8
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More Files
- More Functions
Western Blot
Western blot analysis using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) against Jurkat (1) , HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon, adipocytes tissues using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) .
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) (green) . Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ETS1.
Immunogen
Recombinant protein corresponding to human ETS1.
Host
Mouse
Theoretical MW (kDa)
50.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) against Jurkat (1) , HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon, adipocytes tissues using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) .Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using ETS1 monoclonal antibody, clone 8A8 (Cat # MAB10639) (green) and negative control (purple). -
Gene Info — ETS1
Entrez GeneID
2113Gene Name
ETS1
Gene Alias
ETS-1, EWSR2, FLJ10768
Gene Description
v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Omim ID
164720Gene Ontology
HyperlinkGene Summary
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM
Other Designations
Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1|ets protein|v-ets avian erythroblastosis virus E2 oncogene homolog 1|v-ets avian erythroblastosis virus E26 oncogene homolog 1|v-ets erythroblastosis virus E26 oncogene homolog 1
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Interactome
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Pathway
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Disease
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