EPCAM monoclonal antibody, clone 7E11
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EPCAM.
Immunogen
Recombinant protein corresponding to human EPCAM.
Host
Mouse
Theoretical MW (kDa)
35
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using EPCAM monoclonal antibody, clone 7E11 (Cat # MAB10634) against HTC116 (1) and T-47D (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) , colon cancer (B) , breast cancer (C) and rectal cancer (D) , using EPCAM monoclonal antibody, clone 7E11 (Cat # MAB10634) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPCAM
Entrez GeneID
4072Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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