EIF4E monoclonal antibody, clone 5D11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) against HeLa (1) , HEK293 (2) and K-562 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer (A) , submaxillary tumor (B) , stomach cancer (C) and prostate cancer (D) using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of GC-7901 cells using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) .
Flow Cytometry
Flow cytometric analysis of HeLa cells using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EIF4E.
Immunogen
Recombinant protein corresponding to human EIF4E.
Host
Mouse
Theoretical MW (kDa)
25
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) against HeLa (1) , HEK293 (2) and K-562 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer (A) , submaxillary tumor (B) , stomach cancer (C) and prostate cancer (D) using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) with DAB staining.Immunofluorescence
Immunofluorescence analysis of GC-7901 cells using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) .Flow Cytometry
Flow cytometric analysis of HeLa cells using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) (green) and negative control (purple). -
Gene Info — EIF4E
Entrez GeneID
1977Gene Name
EIF4E
Gene Alias
CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
Gene Description
eukaryotic translation initiation factor 4E
Omim ID
133440Gene Ontology
HyperlinkGene Summary
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
Other Designations
eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
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Interactome
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Pathway
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Disease
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