DLK1 monoclonal antibody, clone 3A10
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) against human DLK1 (aa 174-349) recombinant protein. (Expected MW is 44.9 kDa)
Immunofluorescence
Immunofluorescence analysis of U251 cells using DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) .
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant DLK1.
Immunogen
Recombinant protein corresponding to human DLK1.
Host
Mouse
Theoretical MW (kDa)
41
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) against human DLK1 (aa 174-349) recombinant protein. (Expected MW is 44.9 kDa)Immunofluorescence
Immunofluorescence analysis of U251 cells using DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) .Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using DLK1 monoclonal antibody, clone 3A10 (Cat # MAB10627) (green) and negative control (red). -
Gene Info — DLK1
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Interactome
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Disease
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