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Last updated: 2017/8/13
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DDX4 monoclonal antibody, clone 2F9H5

  • Catalog # : MAB10623
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant DDX4.
  • Immunogen:
  • Recombinant protein corresponding to human DDX4.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 76
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) against DDX4-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) , showing cytoplasmic localization using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of MSCs (upper) and NTERA-2 (bottom) cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MSCs cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) and negative control (purple) .
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • DDX4
  • Gene Alias:
  • MGC111074,VASA
  • Gene Description:
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • Gene Summary:
  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq
  • Other Designations:
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4,OTTHUMP00000122546
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