Mouse monoclonal antibody raised against partial recombinant DDX4.
Recombinant protein corresponding to human DDX4.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) , showing cytoplasmic localization using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) with DAB staining.
Immunofluorescence analysis of MSCs (upper) and NTERA-2 (bottom) cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin.
Flow cytometric analysis of MSCs cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) and negative control (purple) .
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq