DDX4 monoclonal antibody, clone 2F9H5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) , showing cytoplasmic localization using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of MSCs (upper) and NTERA-2 (bottom) cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin.
Flow Cytometry
Flow cytometric analysis of MSCs cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant DDX4.
Immunogen
Recombinant protein corresponding to amino acids 1-160 of human DDX4.
Host
Mouse
Theoretical MW (kDa)
76
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000))
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) against DDX4-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) , showing cytoplasmic localization using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) with DAB staining.Immunofluorescence
Immunofluorescence analysis of MSCs (upper) and NTERA-2 (bottom) cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MSCs cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) and negative control (purple). -
Gene Info — DDX4
Entrez GeneID
54514Gene Name
DDX4
Gene Alias
MGC111074, VASA
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Omim ID
605281Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|OTTHUMP00000122546
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