CRTC1 monoclonal antibody, clone 1B5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CRTC1 monoclonal antibody, clone 1B5 (Cat # MAB10614) against CRTC1-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence
Immunofluorescence analysis of U251 (upper) and NTERA2 (bottom) cells using CRTC1 monoclonal antibody, clone 1B5 (Cat # MAB10614) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of K-562 cells using CRTC1 monoclonal antibody, clone 1B5 (Cat # MAB10614) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CRTC1.
Immunogen
Recombinant protein corresponding to human CRTC1.
Host
Mouse
Theoretical MW (kDa)
67
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CRTC1 monoclonal antibody, clone 1B5 (Cat # MAB10614) against CRTC1-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence analysis of U251 (upper) and NTERA2 (bottom) cells using CRTC1 monoclonal antibody, clone 1B5 (Cat # MAB10614) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using CRTC1 monoclonal antibody, clone 1B5 (Cat # MAB10614) (green) and negative control (purple). -
Gene Info — CRTC1
Entrez GeneID
23373Gene Name
CRTC1
Gene Alias
FLJ14027, KIAA0616, MECT1, TORC1, WAMTP1
Gene Description
CREB regulated transcription coactivator 1
Omim ID
607536Gene Ontology
HyperlinkOther Designations
mucoepidermoid carcinoma translocated 1|transducer of regulated cAMP response element-binding protein (CREB) 1
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Interactome
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Disease
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